Corinne Hrnicek
Dr. Chapman
Phoenix College Stem Train Scholarship
22 October 2024
Project Background
This topic was selected because it is significant in medicine, biology, pharmacology, and toxicology (my intended major when I transfer). Learning more about this topic and researching medicinal plants is essential. An example of medicinal plants' effect on the pharmacology and toxicology field is that most medicines come from plants and have a long history of medicinal plants. It is essential to research this area because we need to know what plants have medicinal properties and if they are toxic to be aware of their uses and dangers. What is currently known about this topic is that Datura has antimicrobial properties and works against Ecoli. It is essential to research this topic further to find the zone of inhibition.
The research will be conducted by observing Datura and experimenting with the plant by extracting medicinal components of the plant to find the medicinal value of Datura. I will then determine if this affects the growth of Ecoli by making a tea and testing it out to see if it affects the growth of Ecoli.
Variable table
Name
I/D/C
Symbol
Units
Description
Plant tea/extract
I
I
?
Plant species (Datura) tea/extract
Growth or no growth of ecoli
D
A
#
Growth or no growth of Ecoli (zone of inhibition)
Using Ecoli for both
Temperature the same
Growth time
C
D
?
Using Ecoli for both
Research question: Does Datura inhibit the growth of Ecoli?
Hypothesis: If we extract the antimicrobial properties of datura, then the growth of Ecoli will be inhibited.
References
Ahsan, Ashraf, et al. "Datura Suaveolens and Verbena Tenuisecta Mediated Silver Nanoparticles, their
Photodynamic Cytotoxic and Antimicrobial Evaluation." World Journal of Microbiology and Biotechnology, vol. 36, no. 2, 2020. ProQuest, https://ezproxy.pc.maricopa.edu/login?url=https://www.proquest.com/scholarly-journals/i-datura-suaveolens-verbena-tenuisecta-mediated/docview/2353365352/se-2, doi:https://doi.org/10.1007/s11274-019-2787-6.
Arage M, Eguale T, Giday M. Evaluation of Antibacterial Activity
and Acute Toxicity of Methanol Extracts of Artemisia absinthium, Datura stramonium, and Solanum anguivi. Infect Drug Resist. 2022 Mar 24;15:1267-1276. doi: 10.2147/IDR.S359280. PMID: 35355620; PMCID: PMC8959718.
Awad MA, Hammad SF, El-Mashtoly SF, El-Deeb B, Soliman HSM. Phytochemical and
biological assessment of secondary metabolites isolated from a rhizosphere strain, Sphingomonas sanguinis DM of Datura metel. BMC Complement Med Ther. 2024 May 25;24(1):205. doi: 10.1186/s12906-024-04482-6. Erratum in: BMC Complement Med Ther. 2024 Jun 18;24(1):237. doi: 10.1186/s12906-024-04543-w. PMID: 38796482; PMCID: PMC11128111.
Benítez, Guillermo, et al. “The genus Datura L. (Solanaceae) in Mexico and Spain
– Ethnobotanical perspective at the interface of medical and illicit uses.” Journal of Ethnopharmacology, vol. 219, 2018, pp. 133–151, https://doi.org/10.1016/j.jep.2018.03.007.
De-la-Cruz IM, Hallab A, Olivares-Pinto U, Tapia-López R, Velázquez-Márquez S,
Piñero D, Oyama K, Usadel B, Núñez-Farfán J. Genomic signatures of the evolution of defense against its natural enemies in the poisonous and medicinal plant Datura stramonium (Solanaceae). Sci Rep. 2021 Jan 13;11(1):882. doi: 10.1038/s41598-020-79194-1. PMID: 33441607; PMCID: PMC7806989.
Rimashree, Devi, et al. "Antimicrobial Activity of Bacterial Endophytes from Chirata (Swertia Chirata
Wall.) and Datura (Datura Stramonium L.)." Egyptian Journal of Biological Pest Control, vol. 31, no. 1, 2021. ProQuest, https://ezproxy.pc.maricopa.edu/login?url=https://www.proquest.com/scholarly-journals/antimicrobial-activity-bacterial-endophytes/docview/2515483836/se-2, doi:https://doi.org/10.1186/s41938-021-00410-9.
Soni P, Siddiqui AA, Dwivedi J, Soni V. Pharmacological properties of Datura
stramonium L. as a potential medicinal tree: an overview. Asian Pac J Trop Biomed. 2012 Dec;2(12):1002-8. doi: 10.1016/S2221-1691(13)60014-3. PMID: 23593583; PMCID: PMC3621465.
Sharma M, Dhaliwal I, Rana K, Delta AK, Kaushik P. Phytochemistry,
Pharmacology, and Toxicology of Datura Species-A Review. Antioxidants (Basel). 2021 Aug 15;10(8):1291. doi: 10.3390/antiox10081291. PMID: 34439539; PMCID: PMC8389218.
Waqar-Ul-Konain M, H Z, Idrees M. Datura innoxia antimicrobial activities against E. coli
isolated from infections of type 2 diabetic patients. Pak J Pharm Sci. 2022 Mar;35(2):479-486. PMID: 35642403.
Yang Y, Dang Y, Li Q, Lu J, Li X, Wang Y. Complete chloroplast genome sequence
of poisonous and medicinal plant Datura stramonium: organizations and implications for genetic engineering. PLoS One. 2014 Nov 3;9(11):e110656. doi: 10.1371/journal.pone.0110656. Erratum in: PLoS One. 2015;10(2):e0118236. Yuanye, Dang [corrected to Dang, Yuanye]; Qing, Li [corrected to Li, Qing]; Jinjian, Lu [corrected to Lu, Jinjian]; Xiwen, Li [corrected to Li, Xiwen]; Yitao, Wang [corrected to Wang, Yitao]. PMID: 25365514; PMCID: PMC4217734.
