Experiment Week 9

Today (Tuesday) was the day we have all been waiting for. All my research has led up to this moment when I did my experiment. I started by heating three beakers with 200 ml water each to 80 degrees Celcius (according to the block heater and 59 degrees Celsius according to the thermometer. Steeped 0.6 g Leaf, 0.3 g Flower, and 1.3 g Stem for 5 min in three separate beakers I put them in an ice bath, then the real fun started. I used a pipet to squeeze two drops of each tea onto separate Petri dishes. I sterilized the tweezers, then used them to get three tiny paper disks out of a small container which was more complicated than I thought. I soaked those separately in the three different Petri dishes and then put those disks in the Ecoli Petri dishes, which I then put in the incubator. I also put a positive control and a negative control in the incubator. I put one petri dish with water instead of the tea to compare it to and another petri dish with a substance known to kill Ecoli instead of the tea.