Corinne Hrnicek
Dr. Chapman
Phoenix College Stem Train program
25 February 2025
Research Proposal
This topic was selected because it is the project stem train students are working on this semester. The DNA extraction kit was sitting on the desk for a long time and now we get to use it. This topic was also selected because it is fascinating and important for medicine, plants, biology, and science. Extracting Plant DNA is important because “It allows scientists to analyze specific genome regions that are conserved across species. It is important to learn more about this topic, because It forms the basis for many downstream applications in scientific research, conservation efforts, and agricultural advancements. It is important to conduct research in this area because It helps study trait inheritance, develop genetically modified organisms (GMOs), and preserve endangered plant species. It is crucial for a wide range of applications in plant biology. It helps identify interesting mutations in plant genes, which can improve or hinder their growth in specific conditions.” (The DNA Connection 2024)
I will research the topic by observing how the DNA extraction tool works and experimenting with the Plant DNA extraction tool. This is my first time using the machine, so observing and experimenting will be a learning experience. (Comparison 2013)
Variable table
Name
I/D/C
Symbol
Units
Description
Plant
I
I
Mg
Plant species extract
DNA extraction results
D
ZOI
#
DNA extraction results
Using the same machine
C
?
Using the same formula
Hypothesis
Hypothesis: The efficiency of DNA extraction from plant tissues varies significantly depending on the type of plant tissue used (e.g., leaves, stems, roots). (Skage, 2007)
Research Question
Research Question: How does the type of plant tissue (leaves, stems, roots) affect the yield and purity of extracted DNA?
I expect to find results that support the hypothesis. The efficiency will vary based on the type of plant tissue. Each DNA sample will be a little different. I expect to learn more about DNA, plant extraction, and the tools used to extract the DNA. (Selz, 2023)
References
The DNA connection: Understanding the significance of plant DNA extraction. Pure
Natural Plant Extracts Manufacturer - Over 19 Years. (2024, August 14). https://www.greenskybio.com/plant_extract/the-dna-connection-understanding-the-significance-of-plant-dna-extraction.html
Selz, J., Adam, N. R., Magrini, C. E. M., Montandon, F. M., Buerki, S., & Maerkl, S. J.
(2023). A field‐capable rapid plant DNA extraction protocol using microneedle patches for botanical surveying and monitoring. Applications in Plant Sciences, 11(3), e11529-n/a. https://doi.org/10.1002/aps3.11529
A Modified CTAB Protocol for Plant DNA Extraction. (n.d.). Zhiwu Xuebao.
https://doi.org/10.3724/sp.j.1259.2013.00072
Skage, M., & Schander, C. (2007). DNA from formalin-fixed tissue - extraction or repair? That
is the question. Marine Biology Research, 3(5).
Comparison of the efficiency of some of the most usual DNA extraction methods for woody
plants in different tissues of Vitis vinifera L. (2013). OENO One. https://doi.org/10.20870/oeno-one.2013.47.4.1559
